Overview

  • Product name
  • Description
    Rabbit polyclonal to Cyclin E1
  • Tested applications
    Suitable for: Functional Studies, ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide from the C-terminal of human cyclin E.

  • Positive control
    • SKBR3 and MAD109 cells. Breast carcinoma or placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab74276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
ICC/IF
IP
WB
IHC-P
  • Application notes
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 47 kDa.
    Kinase Assay: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Essential for the control of the cell cycle at the G1/S (start) transition.
    • Tissue specificity
      Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • Sequence similarities
      Belongs to the cyclin family. Cyclin E subfamily.
    • Post-translational
      modifications
      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    Images

    • ab74276, at 1/50 dilution, staining Cyclin E in formalin-fixed, paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.

    References

    ab74276 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hela)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    Hela
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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