Overview

  • Product nameAnti-Cyclin E1 antibody
    See all Cyclin E1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin E1
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin E1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab101079, ab101079)

  • Positive control
    • This antibody gave a positive signal in HepG2 whole cell lysate and in the following mouse tissue lysates: Brain; Testis; Heart. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab88259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
  • Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
  • Post-translational
    modifications
    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody images

  • IHC image of Cyclin E staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88259, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Cyclin E1 antibody (ab88259) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 32 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • ICC/IF image of ab88259 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88259, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

References for Anti-Cyclin E1 antibody (ab88259)

This product has been referenced in:
  • Bonazzi VF  et al. Cross-Platform Array Screening Identifies COL1A2, THBS1, TNFRSF10D and UCHL1 as Genes Frequently Silenced by Methylation in Melanoma. PLoS One 6:e26121 (2011). WB ; Human . Read more (PubMed: 22028813) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Butyl-Methyl Methacrylate 2micron Cornea Sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Specification Butyl-Methyl Methacrylate 2micron Cornea Sections
Blocking step Serum as blocking agent for 2 minute(s) · Concentration: 5%
Fixative Paraformaldehyde
Username

Geraint Parfitt

Verified customer

Submitted Mar 08 2016

Application IHC - Wholemount
Sample Fruit fly (Drosophila melanogaster) Tissue (Brain)
Specification Brain
Username

Abcam user community

Verified customer

Submitted Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"