Overview

  • Product nameAnti-Cyclin E1 antibody
    See all Cyclin E1 primary antibodies
  • Description
    Mouse polyclonal to Cyclin E1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Cyclin E1.

  • Positive control
    • Human colon tissue lysate, Cyclin E1 transfected 293T cell line lysate and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab89585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
  • Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
  • Post-translational
    modifications
    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody images

  • Anti-Cyclin E1 antibody (ab89585) at 1 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • All lanes : Anti-Cyclin E1 antibody (ab89585) at 1 µg/ml

    Lane 1 : Cyclin E transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • ab89585 at 10 µg/ml staining Cyclin E in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.

References for Anti-Cyclin E1 antibody (ab89585)

ab89585 has not yet been referenced specifically in any publications.

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