Anti-Cyclin E1 (phospho T77) antibody (ab62199)

Overview

  • Product nameAnti-Cyclin E1 (phospho T77) antibody
    See all Cyclin E1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin E1 (phospho T77)
  • Specificityab62199 detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 77.
  • Tested applicationsSuitable for: ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin E1.

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

Target

  • FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
  • Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
  • Post-translational
    modifications
    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 (phospho T77) antibody images

  • Immunofluorescence analysis of HeLa cells using ab62199 at a dilution of 1/500. Samples were untreated (left hand panel) or treated (right hand panel) with the immunizing peptide.

References for Anti-Cyclin E1 (phospho T77) antibody (ab62199)

ab62199 has not yet been referenced specifically in any publications.

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