Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696)

Overview

  • Product nameAnti-Cyclin E1 (phospho T77) antibody [EPR2242(2)]
    See all Cyclin E1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2242(2)] to Cyclin E1 (phospho T77)
  • Specificityab124696 only detects Cyclin E1 phosphorylated at Threonine 77.
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to residues surrounding Threonine 77 of Human Cyclin E1.

  • Positive control
    • JAR cell lysate, treated with Lambda Phosphatase; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
    • Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
    • Post-translational
      modifications
      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] images

    • All lanes : Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696) at 1/1000 dilution

      Lane 1 : JAR cell lysate, untreated
      Lane 2 : JAR cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 47 kDa
    • ab124696, at 1/100, staining Cyclin E1 (phospho T77) in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696)

    ab124696 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"