The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/250 - 1/500.
Application notesIs unsuitable for IP.
FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
Post-translational modificationsPhosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.