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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
1/50 - 1/100.
1/500 - 1/1000.
Relevance The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
Cellular localization Nuclear
CCN E2 antibody
CCNE 2 antibody
Anti-Cyclin E2 antibody images
Western blot - Cyclin E2 antibody (ab78941)
All lanes : Anti-Cyclin E2 antibody (ab78941) at 1/500 dilution Lane 1 : Extracts from COLO cells Lane 2 : Extracts from
Jurkat cells Lane 3 : Extracts from COLO cells with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 47 kDa Observed band size : 47 kDa Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin E2 antibody (ab78941)
Immunohistochemistry analysis of paraffin-embedded
human thyroid gland tissue using ab78941 at 1/50 dilution in the absence (left panel) or presence (right panel) of immunising peptide.
Immunocytochemistry/ Immunofluorescence - Cyclin E2 antibody (ab78941)
Immunofluorescence analysis of NIH/3T3 cells using
ab78941 at 1/500 dilution in the absence (left panel) or presence (right panel) of immunising peptide.
References for Anti-Cyclin E2 antibody (ab78941)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"