Recombinant
RabMAb

Anti-Cyclin E2 antibody [EP454Y] (ab40890)

Overview

  • Product name
    Anti-Cyclin E2 antibody [EP454Y]
    See all Cyclin E2 primary antibodies
  • Description
    Rabbit monoclonal [EP454Y] to Cyclin E2
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: Jurkat cell lysate. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma. Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Cyclin E2 antibody (Alexa Fluor® 488) [EP454Y] (ab200423)

    Anti-Cyclin E2 antibody (Alexa Fluor® 647) [EP454Y] (ab200424)

    Anti-Cyclin E2 antibody (HRP) [EP454Y] (ab200425)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab40890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/230.
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/250 - 1/500.
ICC/IF 1/100 - 1/250.
IP 1/50.

Target

  • Relevance
    The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • CCN E2 antibody
    • CCNE 2 antibody
    • CCNE2 antibody
    • CCNE2 protein antibody
    • CYC E2 antibody
    • CYCE 2 antibody
    • CYCE2 antibody
    • CyclinE2 antibody
    • G1/S specific cyclin E2 antibody
    see all

Images

  • Anti-Cyclin E2 antibody [EP454Y] (ab40890) at 1/2000 dilution + Jurkat cell lysate at 10 µg

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • Immunofluorescent staining of staining HeLa cells using ab40890 (1/100).
  • ab40890 (1/250) staining human breast carcinoma by immunohistochemistry, paraffin-embedded tissue.
  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cyclin E2 (right) with ab40890 at a 1/230 dilution. Cells were fixed with 90% ethanol and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution.

    Left panel - Rabbit monoclonal IgG (ab172730).

References

This product has been referenced in:
  • Song W  et al. PARP inhibitor increases chemosensitivity by upregulating miR-664b-5p in BRCA1-mutated triple-negative breast cancer. Sci Rep 7:42319 (2017). Read more (PubMed: 28176879) »
  • Fischer MM  et al. WNT antagonists exhibit unique combinatorial antitumor activity with taxanes by potentiating mitotic cell death. Sci Adv 3:e1700090 (2017). Read more (PubMed: 28691093) »

See all 12 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 06 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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