The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/20 - 1/40. High temperature antigen unmasking technique. 60 minutes primary antibody incubation at 25oC and standard ABC technique are recommended.
WB: 1/100 - 1/250. Predicted molecular weight: 34 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation.
Tissue specificityHigh levels in skeletal muscle, ovary, kidney and colon.
Sequence similaritiesBelongs to the cyclin family. Cyclin G subfamily.
Developmental stageVery low levels in normal cells during G1 phase, which increase as cells enter the S phase and stay high throughout the S and G2/M phases. In breast cancer cells consistent high levels are found throughout the cell cycle.
Cellular localizationNucleus. DNA replication foci after DNA damage.