Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab5502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21423799
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May play a role in growth regulation and in negative regulation of cell cycle progesssion.
  • Tissue specificity
    High levels in cerebellum, thymus, spleen and prostate. Low levels in skeletal muscle.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin G subfamily.
  • Developmental stage
    Expression levels increase through the cell cycle to peak in the mid/late-S phase and decrease during G2/M phase.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNG 2 antibody
    • Ccng2 antibody
    • CCNG2 protein antibody
    • CCNG2_HUMAN antibody
    • Cyclin-G2 antibody
    • CyclinG2 antibody
    see all

Anti-Cyclin G2 antibody images



  • Predicted band size : 39 kDa


    ab5502 at a 1/1000 dilution staining approximately 39 kDa Cyclin G2 in mouse muscle extract by Western blot (ECL).

References for Anti-Cyclin G2 antibody (ab5502)

This product has been referenced in:
  • Fujimura A  et al. Expression of a constitutively active calcineurin encoded by an intron-retaining mRNA in follicular keratinocytes. PLoS One 6:e17685 (2011). WB, IHC-Fr ; Human . Read more (PubMed: 21423799) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mammary)
Specification
Mammary
Fixative
Paraformaldehyde
Permeabilization
Yes - Citrate pH6 antigen retrieval + 0.025% Triton X permeabilization
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 01 2011

I understand your concern regarding the non specific bands in the myoblast cell lysate and the human cancer cell line lysate. Cancer cell lines can undergo different morphological, biochemical and molecular biological changes especially after certain p...

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Thank you for your enquiry and for taking your time to fill in the Questionnaire. We are very sorry to hear that you are having problem with this antibody. 1. Ab5502 has been tested and characterised for Western blot application using mouse muscle ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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