Overview

  • Product nameAnti-Cyclin H antibody
    See all Cyclin H primary antibodies
  • Description
    Mouse polyclonal to Cyclin H
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Cyclin H aa 1-323.

  • Positive control
    • human kidney lysate, transfected 293T cell line, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab89257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37.6 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionRegulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6330408H09Rik antibody
    • AI661354 antibody
    • AV102684 antibody
    • AW538719 antibody
    • CAK antibody
    • CAK complex subunit antibody
    • ccnh antibody
    • CCNH_HUMAN antibody
    • CDK activating kinase antibody
    • CDK activating kinase complex subunit antibody
    • Cyclin dependent kinase activating kinase antibody
    • cyclin dependent kinase activating kinase complex subunit antibody
    • Cyclin H antibody
    • Cyclin-H antibody
    • CyclinH antibody
    • MO15 associated protein antibody
    • MO15-associated protein antibody
    • p34 antibody
    • p36 antibody
    • p37 antibody
    see all

Anti-Cyclin H antibody images

  • Anti-Cyclin H antibody (ab89257) at 5 µg/ml + human kidney lysate at 50 µg

    Secondary
    HRP goat anti mouse IgG at 1/2500 dilution

    Predicted band size : 37.6 kDa
  • All lanes : Anti-Cyclin H antibody (ab89257) at 5 µg/ml

    Lane 1 : transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP goat anti mouse IgG at 1/2500 dilution

    Predicted band size : 37.6 kDa
  • Immunofluorescence analysis of CCR1 expression in HeLa cells using ab89257 at 10 ug/ml

References for Anti-Cyclin H antibody (ab89257)

ab89257 has not yet been referenced specifically in any publications.

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