Anti-Cyclin H (phospho T315) antibody (ab73208)

Overview

  • Product name
    Anti-Cyclin H (phospho T315) antibody
    See all Cyclin H primary antibodies
  • Description
    Rabbit polyclonal to Cyclin H (phospho T315)
  • Specificity
    ab73208 detects endogenous levels of Cyclin H only when phosphorylated at threonine 315.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Cyclin H around the phosphorylation site of threonine 315 (EWTPDD)

  • Positive control
    • HeLa cell extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab73208 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
ELISA 1/10000.

Target

  • Function
    Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin C subfamily.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6330408H09Rik antibody
    • AI661354 antibody
    • AV102684 antibody
    • AW538719 antibody
    • CAK antibody
    • CAK complex subunit antibody
    • ccnh antibody
    • CCNH_HUMAN antibody
    • CDK activating kinase antibody
    • CDK activating kinase complex subunit antibody
    • Cyclin dependent kinase activating kinase antibody
    • cyclin dependent kinase activating kinase complex subunit antibody
    • Cyclin H antibody
    • Cyclin-H antibody
    • CyclinH antibody
    • MO15 associated protein antibody
    • MO15-associated protein antibody
    • p34 antibody
    • p36 antibody
    • p37 antibody
    see all

Images

  • All lanes : Anti-Cyclin H (phospho T315) antibody (ab73208) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 38 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

References

ab73208 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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