Overview

  • Product name
  • Description
    Mouse polyclonal to Cyclin M3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human)

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab67034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    Note: ab67034 required Protein A affinity purification prior to ICC/IF application.
    WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 77 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Cyclin M3 is a probable metal transporter. There are three named isoforms.
    • Cellular localization
      Integral to plasma membrane; Multi-pass membrane protein
    • Database links
    • Alternative names
      • ACDP3 antibody
      • Ancient conserved domain containing protein 3 antibody
      • CNNM3 antibody
      • DKFZp434I1016 antibody
      • FLJ20018 antibody
      • Metal transporter CNNM3 antibody
      see all

    Anti-Cyclin M3 antibody images

    • All lanes : Anti-Cyclin M3 antibody (ab67034) at 1/500 dilution

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 77 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescence of purified ab67034 to Cyclin M3 on HeLa cells. Antibody concentration 10 ug/ml.

    References for Anti-Cyclin M3 antibody (ab67034)

    ab67034 has not yet been referenced specifically in any publications.

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