Anti-Cyclin T1 antibody (ab106968)

Overview

  • Product nameAnti-Cyclin T1 antibody
    See all Cyclin T1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin T1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within internal sequence amino acids 256-285 of Human Cyclin T1 (NP_001231).

  • Positive control
    • MCF-7 cell line lysate; Mouse testis tissue lysate; Human prostate carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab106968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 81 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN T1 antibody
    • CCNT antibody
    • CCNT 1 antibody
    • Ccnt1 antibody
    • CCNT1_HUMAN antibody
    • CDK9 associated C type protein antibody
    • Cyc T1 antibody
    • Cyclin C related protein antibody
    • Cyclin T antibody
    • cyclin T1 antibody
    • Cyclin T1b antibody
    • Cyclin-T antibody
    • Cyclin-T1 antibody
    • CYCT 1 antibody
    • CycT1 antibody
    • HIVE1 antibody
    • Human immunodeficiency virus 1 expression antibody
    • Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody
    • pTEFb subunit antibody
    • Subunit of positive elongation transcription factor b antibody
    see all

Anti-Cyclin T1 antibody images

  • Anti-Cyclin T1 antibody (ab106968) at 1/100 dilution + MCF-7 cell line lysate at 35 µg

    Predicted band size : 81 kDa
  • Anti-Cyclin T1 antibody (ab106968) at 1/100 dilution + Mouse testis tissue lysate at 35 µg

    Predicted band size : 81 kDa
  • ab106968, at 1/50 dilution, staining Cyclin T1 in formalin fixed, paraffin embedded Human prostate carcinoma by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References for Anti-Cyclin T1 antibody (ab106968)

ab106968 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Nov 19 2012

Thank you for your reply. I am sorry to hear that the results are still not satisfactory.
Your credit note ID is ***
I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future o...

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Thank you for contacting us and for letting us know about the trouble with this antibody.
The protocol that you have used looks fine, but I would recommend trying up to 1:100 dilution of antibody which has worked well in other testing. We do fully...

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