Overview

  • Product name
  • Description
    Sheep polyclonal to Cyclin T1
  • Tested applications
    Suitable for: WB, IP, EMSAmore details
  • Species reactivity
    Reacts with: Mouse, Horse, Human, Monkey
    Does not react with: Drosophila melanogaster
  • Immunogen

    Recombinant fragment, corresponding to C terminal amino acids 291-726 of Human Cyclin T1

  • General notes


    ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.

Properties

Applications

Our Abpromise guarantee covers the use of ab27963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 81 kDa).
IP Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration. PubMed: 17576689

Target

  • Function
    Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin C subfamily.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN T1 antibody
    • CCNT antibody
    • CCNT 1 antibody
    • Ccnt1 antibody
    • CCNT1_HUMAN antibody
    • CDK9 associated C type protein antibody
    • Cyc T1 antibody
    • Cyclin C related protein antibody
    • Cyclin T antibody
    • cyclin T1 antibody
    • Cyclin T1b antibody
    • Cyclin-T antibody
    • Cyclin-T1 antibody
    • CYCT 1 antibody
    • CycT1 antibody
    • HIVE1 antibody
    • Human immunodeficiency virus 1 expression antibody
    • Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody
    • pTEFb subunit antibody
    • Subunit of positive elongation transcription factor b antibody
    see all

Anti-Cyclin T1 antibody images

  • All lanes : Anti-Cyclin T1 antibody (ab27963) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa cell nuclear lysate
    Lane 3 : monkey Vero cell lysate
    Lane 4 : mouse kidney cell lysate
    Lane 5 : Drosophila S2 cell lysate
    Lane 6 : horse dermal cell lysate

    Secondary
    donkey anti-sheep peroxidase conjugate at 1/20000 dilution

    Predicted band size : 81 kDa
    Observed band size : 87 kDa (why is the actual band size different from the predicted?)

References for Anti-Cyclin T1 antibody (ab27963)

This product has been referenced in:
  • Brogie JE & Price DH Reconstitution of a functional 7SK snRNP. Nucleic Acids Res 45:6864-6880 (2017). Read more (PubMed: 28431135) »
  • Krueger BJ  et al. LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated. Nucleic Acids Res : (2008). WB ; Human . Read more (PubMed: 18281698) »

See all 3 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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