Anti-Cyclin T2/CCNT2 antibody [2128C1a] (ab50979)
Key features and details
- Mouse monoclonal [2128C1a] to Cyclin T2/CCNT2
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Cyclin T2/CCNT2 antibody [2128C1a]
See all Cyclin T2/CCNT2 primary antibodies -
Description
Mouse monoclonal [2128C1a] to Cyclin T2/CCNT2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Cyclin T2/CCNT2 (C terminal).
Database link: O60583 -
Epitope
ab50979 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
General notes
This antibody recognizes Cyclin T2a and T2b since it is made by immunogen-peptide that is common domain from both protein.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2128C1a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa. |
Target
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Function
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the cyclin family. Cyclin C subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 905 Human
- Omim: 603862 Human
- SwissProt: O60583 Human
- Unigene: 658324 Human
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Alternative names
- CCNT2 antibody
- CCNT2_HUMAN antibody
- Cyclin T2a antibody
see all
Images
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Anti-Cyclin T2/CCNT2 antibody [2128C1a] (ab50979) + recombinant protein fragment used as immunogen.
Diagram underneath blot depicts region of Cyclin T2 protein that was employed as immunogen (arrow).
Predicted band size: 81 kDa -
All lanes : Anti-Cyclin T2/CCNT2 antibody [2128C1a] (ab50979)
Lane 1 : Molecular Weight Marker
Lane 2 : 50µg Hela Whole cell lysate
Lane 3 : 50µg HT-1080 Whole cell lysate
Lane 4 : 50 µg HEK293 Whole cell lysate
Lane 5 : 50µg A2058 Whole cell lysate
Lane 6 : 50µg HIH3T3 Whole cell Lysate
Secondary
Lanes 2-6 : Mouse IgG Antibody 1/2500
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Exposure time: 1 minute
8% SDS-PAGE gel;
a- Isoform a = 74kDa (663 a.a.);
b - Isoform b = 81kDa (730 a.a.)
Datasheets and documents
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Datasheet download
References (3)
ab50979 has been referenced in 3 publications.
- Donnio LM et al. CSB-Dependent Cyclin-Dependent Kinase 9 Degradation and RNA Polymerase II Phosphorylation during Transcription-Coupled Repair. Mol Cell Biol 39:N/A (2019). PubMed: 30602496
- Zhang H et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). PubMed: 23898190
- Wang XS et al. MicroRNA-29a and microRNA-142-3p are regulators of myeloid differentiation and acute myeloid leukemia. Blood 119:4992-5004 (2012). WB ; Human . PubMed: 22493297