Overview

  • Product name
    Anti-Cyclin T2 antibody [2128C1a]
    See all Cyclin T2 primary antibodies
  • Description
    Mouse monoclonal [2128C1a] to Cyclin T2
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment designed around the C-terminal end of human Cyclin T2.

  • Epitope
    ab50979 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • General notes
    This antibody recognizes Cyclin T2a and T2b since it is made by immunogen-peptide that is common domain from both protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8.0mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    2128C1a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 81 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II).
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin C subfamily.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNT2 antibody
    • CCNT2_HUMAN antibody
    • Cyclin T2a antibody
    • Cyclin T2b antibody
    • Cyclin-T2 antibody
    • CycT2 antibody
    • FLJ90560 antibody
    • MGC134840 antibody
    • SDS stable vimentin bound DNA fragment HEF42VIM22 antibody
    • Subunit of positive elongation transcription factor b antibody
    see all

Anti-Cyclin T2 antibody [2128C1a] images

  • Anti-Cyclin T2 antibody [2128C1a] (ab50979) + recombinant protein fragment used as immunogen.
    Diagram underneath blot depicts region of Cyclin T2 protein that was employed as immunogen (arrow).

    Predicted band size : 81 kDa
  • All lanes : Anti-Cyclin T2 antibody [2128C1a] (ab50979)

    Lane 1 : Molecular Weight Marker
    Lane 2 : 50µg Hela Whole cell lysate
    Lane 3 : 50µg HT-1080 Whole cell lysate
    Lane 4 : 50 µg HEK293 Whole cell lysate
    Lane 5 : 50µg A2058 Whole cell lysate
    Lane 6 : 50µg HIH3T3 Whole cell Lysate

    Secondary
    Lanes 2 - 6 : Mouse IgG Antibody 1/2500

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 81 kDa


    Exposure time : 1 minute

References for Anti-Cyclin T2 antibody [2128C1a] (ab50979)

This product has been referenced in:
  • Zhang H  et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). Read more (PubMed: 23898190) »
  • Wang XS  et al. MicroRNA-29a and microRNA-142-3p are regulators of myeloid differentiation and acute myeloid leukemia. Blood 119:4992-5004 (2012). WB ; Human . Read more (PubMed: 22493297) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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