Overview

  • Product nameAnti-Cyclin T2 antibody [2128C1a]
    See all Cyclin T2 primary antibodies
  • Description
    Mouse monoclonal [2128C1a] to Cyclin T2
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment designed around the C-terminal end of human Cyclin T2.

  • Epitopeab50979 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • General notesThis antibody recognizes Cyclin T2a and T2b since it is made by immunogen-peptide that is common domain from both protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8.0mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number2128C1a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 81 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II).
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNT2 antibody
    • CCNT2_HUMAN antibody
    • Cyclin T2a antibody
    • Cyclin T2b antibody
    • Cyclin-T2 antibody
    • CycT2 antibody
    • FLJ90560 antibody
    • MGC134840 antibody
    • SDS stable vimentin bound DNA fragment HEF42VIM22 antibody
    • Subunit of positive elongation transcription factor b antibody
    see all

Anti-Cyclin T2 antibody [2128C1a] images

  • Anti-Cyclin T2 antibody [2128C1a] (ab50979) + recombinant protein fragment used as immunogen.
    Diagram underneath blot depicts region of Cyclin T2 protein that was employed as immunogen (arrow).

    Predicted band size : 81 kDa
  • All lanes : Anti-Cyclin T2 antibody [2128C1a] (ab50979)

    Lane 1 : Molecular Weight Marker
    Lane 2 : 50µg Hela Whole cell lysate
    Lane 3 : 50µg HT-1080 Whole cell lysate
    Lane 4 : 50 µg HEK293 Whole cell lysate
    Lane 5 : 50µg A2058 Whole cell lysate
    Lane 6 : 50µg HIH3T3 Whole cell Lysate

    Secondary
    Lanes 2 - 6 : Mouse IgG Antibody 1/2500

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 81 kDa


    Exposure time : 1 minute

References for Anti-Cyclin T2 antibody [2128C1a] (ab50979)

This product has been referenced in:
  • Zhang H  et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). Read more (PubMed: 23898190) »
  • Wang XS  et al. MicroRNA-29a and microRNA-142-3p are regulators of myeloid differentiation and acute myeloid leukemia. Blood 119:4992-5004 (2012). WB ; Human . Read more (PubMed: 22493297) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab50979.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"