Overview

  • Product nameAnti-Cyclophilin A antibody
    See all Cyclophilin A primary antibodies
  • Description
    Rabbit polyclonal to Cyclophilin A
  • SpecificityDetects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Human
    Predicted to work with: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African Green Monkey
  • Immunogen

    Other Immunogen Type corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.

Properties

Applications

Our Abpromise guarantee covers the use of ab3563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/300.
ELISA 1/1000.

Used for 2 hrs on Rabbit liver cells.

ICC Use at an assay dependent concentration.

Target

Anti-Cyclophilin A antibody images

  • ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).

  • ICC/IF image of ab3563 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3563, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : NIH-3T3

    Lysates/proteins at 25 µg per lane.


    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

References for Anti-Cyclophilin A antibody (ab3563)

This product has been referenced in:
  • Fan YN  et al. Mir-34a Mimics Are Potential Therapeutic Agents for p53-Mutated and Chemo-Resistant Brain Tumour Cells. PLoS One 9:e108514 (2014). WB . Read more (PubMed: 25250818) »
  • Lorenson MY  et al. A major prolactin-binding complex on human milk fat globule membranes contains cyclophilins A and B: the complex is not the prolactin receptor. Am J Physiol Endocrinol Metab 302:E585-94 (2012). WB ; Human . Read more (PubMed: 22205628) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MeT5a)
Loading amount 15 µg
Specification MeT5a
Gel Running Conditions Reduced Denaturing (10% Acrylamide, Laemmli buffer system)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2008

Thank you for your enquiry. This product ab3563 is supplied as 400 µl of total IgG in PBS containing 0.05% sodium azide. Unfortunately, the concentration of this product has not been determined at this time, as the antibody has only been protein (a...

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Thank you for your enquiry. This antibody is listed on the data sheet as being an IgG fraction. Because this antibody is not affinity purified, the concentration is undetermined. I understand that you need the concentration for your assays, but unfortu...

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Thank you for your enquiry. Regarding polyclonal antibody to Cyclophilin A ab3563, our source has not measured the IgG concentration. The activity of some of the antibodies we sell is based on ELISA data. However,the concentration is expected to be...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Purified protein (competitive ELISA assay)
Specification competitive ELISA assay
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Type Other
Username

Abcam user community

Verified customer

Submitted Jul 31 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rabbit Cell (rabbit liver cells)
Specification rabbit liver cells
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2
Type Other
Username

Abcam user community

Verified customer

Submitted Jul 28 2006

Thank you for your enquiry. Cyclophilin A is described as a housekeeoping gene in the literature. However, this cyclophilin A antibody does not detect endogenous levels of CyPA, so it would not be suitable for this purpose. I hope this informati...

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I apologize, but it looks like the IgG concentration is unknown for ab3563 (Cyclophilin A). Occasionally, ELISA tests are used to determine activity instead of measuring the protein concentration. More than likely, the range falls between 0.5-2 mg/ml. ...

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I'm sorry to hear you are experiencing problems with ab3563. I would like to recommend incubating the membrane in the antibody solution overnight at 4C. Please let me know if you still have problems following this recommendation and I will arrange a...

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The cyclophilin A antibody (ab3563) has not been tested for IP, currently we have no other cyclophilin A antibody that has. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"