Overview

  • Product name
    Anti-Cyclophilin A antibody
    See all Cyclophilin A primary antibodies
  • Description
    Rabbit polyclonal to Cyclophilin A
  • Specificity
    Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Human
    Predicted to work with: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African green monkey
  • Immunogen

    Other Immunogen Type corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.

Properties

Applications

Our Abpromise guarantee covers the use of ab3563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/300.
ELISA 1/1000.

Used for 2 hrs on Rabbit liver cells.

ICC Use at an assay dependent concentration.

Target

Images

  • ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).

  • ICC/IF image of ab3563 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3563, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : NIH-3T3

    Lysates/proteins at 25 µg per lane.


    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Fan YN  et al. Mir-34a Mimics Are Potential Therapeutic Agents for p53-Mutated and Chemo-Resistant Brain Tumour Cells. PLoS One 9:e108514 (2014). WB . Read more (PubMed: 25250818) »
  • Lorenson MY  et al. A major prolactin-binding complex on human milk fat globule membranes contains cyclophilins A and B: the complex is not the prolactin receptor. Am J Physiol Endocrinol Metab 302:E585-94 (2012). WB ; Human . Read more (PubMed: 22205628) »

See all 5 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Western blot
Sample
Mouse Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
skin
Blocking step
licor fluorescent blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
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Abcam user community

Verified customer

Submitted Nov 23 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MeT5a)
Loading amount
15 µg
Specification
MeT5a
Gel Running Conditions
Reduced Denaturing (10% Acrylamide, Laemmli buffer system)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Purified protein (competitive ELISA assay)
Specification
competitive ELISA assay
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Type
Other
Username

Abcam user community

Verified customer

Submitted Jul 31 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rabbit Cell (rabbit liver cells)
Specification
rabbit liver cells
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2
Type
Other
Username

Abcam user community

Verified customer

Submitted Jul 28 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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