Overview

  • Product nameAnti-Cyclophilin A antibody
    See all Cyclophilin A primary antibodies
  • Description
    Rabbit polyclonal to Cyclophilin A
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig, Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Cyclophilin A.

    (Peptide available as ab42406.)

  • Positive control
    • This antibody gave a positive signal in the following human lysates: HeLa (Human epithelial carcinoma cell line) whole cell A431 (Human epithelial carcinoma cell line) whole cell Lymph node (Human) whole cell - adult normal tissue Thymus tissue This antibody gave a positive signal in the following rat lysate: Lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab41684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: Use at a dilution of 1/100.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Cyclophilin A antibody images

    • All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
      Lane 4 : Human thymus tissue lysate - total protein (ab30146)
      Lane 5 : Lung (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
    • ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • ab41684 staining mouse heart and rat thymus tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C.  The primay antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C.  A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.

      See Abreview

    • Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml + Cyclophilin A protein (Human) (ab88202) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    References for Anti-Cyclophilin A antibody (ab41684)

    This product has been referenced in:
    • Zhang J  et al. Pyrrolidine dithiocarbamate attenuates surgery-induced neuroinflammation and cognitive dysfunction possibly via inhibition of nuclear factor ?B. Neuroscience 261:1-10 (2014). Rat . Read more (PubMed: 24365462) »
    • Kim HC  et al. Quantitative ultrasound characterization of tumor cell death: ultrasound-stimulated microbubbles for radiation enhancement. PLoS One 9:e102343 (2014). IHC-P ; Mouse . Read more (PubMed: 25051356) »

    See all 10 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Dog Tissue sections (Skin)
    Antigen retrieval step None
    Permeabilization No
    Specification Skin
    Blocking step (agent) for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HepaRG)
    Specification HepaRG
    Fixative Formaldehyde
    Permeabilization Yes - 0.2% Triton X-100 in PBS
    Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Mrs. Alina Macovei

    Verified customer

    Submitted May 03 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HepaRG)
    Loading amount 10 µg
    Specification HepaRG
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Username

    Mrs. Alina Macovei

    Verified customer

    Submitted May 03 2012

    Thank you for your message and for confirming these details. I am very pleased to hear the customer would like to accept our offer and test these antibodies in hamster. ab41684 Discount code: #### ab38975 Discount code: #### ab6314 ...

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    Thank you for your message which has been forwarded to me as Karin is currently away from the office. I have reviewed these three antibodies with respect to discount codes: ab77179 I am sorry I am also unable to check the alignment of the immun...

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    Thank you for contacting us. If an antibody has not been tested with a particular species then a testing discount would apply. However, in order to check the likelihood of the antibody being reactive against the target the hamster protein sequence need...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293T)
    Loading amount 20 µg
    Specification 293T
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Pankaj Gupta

    Verified customer

    Submitted Sep 01 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (mouse (heart), rat (thymus))
    Specification mouse (heart), rat (thymus)
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization No
    Blocking step Sequential peroxidase and protein block (prediluted) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 03 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (MEF, NIH-3T3, RAW)
    Loading amount 20 µg
    Specification MEF, NIH-3T3, RAW
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 01 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"