Overview

  • Product nameAnti-Cyclophilin A antibody
    See all Cyclophilin A primary antibodies
  • Description
    Rabbit polyclonal to Cyclophilin A
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Dog, Pig, Chimpanzee, Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 of Human Cyclophilin A.

    (Peptide available as ab42407.)

  • Positive control
    • This antibody gave a positive signal in the following human lysates: HeLa (Human epithelial carcinoma cell line) whole cell A431 (Human epithelial carcinoma cell line) whole cell Lymph node (Human) whole cell - adult normal tissue Thymus tissue This antibody gave a positive signal in the following rat lysate: Lung tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab42408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: Use at a concentration of 5 µg/ml.
    IHC-P: Use at in the range of 1/100 - 1/500 depending on incubation time/ condtions of primary.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Cyclophilin A antibody images

    • All lanes : Anti-Cyclophilin A antibody (ab42408) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
      Lane 4 : Human thymus tissue lysate - total protein (ab30146)
      Lane 5 : Lung (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
    • Anti-Cyclophilin A antibody (ab42408) at 1/1000 dilution + Recombinant Human Cyclophilin A protein (ab96022) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute
    • ICC/IF image of ab42408 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab42408, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • ab42408 staining Cyclophilin A in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval and then blocked (5 minutes of peroxidase block and 10 minutes of protein block) for 15 minutes at 20°C. The primary antibody was used undiluted and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used, undiluted as secondary.

      See Abreview

    References for Anti-Cyclophilin A antibody (ab42408)

    This product has been referenced in:
    • Heinzmann D  et al. The Novel Extracellular Cyclophilin A (CyPA) - Inhibitor MM284 Reduces Myocardial Inflammation and Remodeling in a Mouse Model of Troponin I -Induced Myocarditis. PLoS One 10:e0124606 (2015). Histology ; Mouse . Read more (PubMed: 25894208) »
    • Keyes LR  et al. Cyclophilin A is required for efficient human cytomegalovirus DNA replication and reactivation. J Gen Virol 93:722-32 (2012). WB ; Human . Read more (PubMed: 22238232) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution
    Permeabilization No
    Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
    Username

    Abcam user community

    Verified customer

    Submitted Sep 16 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (heart)
    Specification heart
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 07 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Heart)
    Specification Heart
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 07 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (heart)
    Specification heart
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 01 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (mouse muscle cells)
    Loading amount 50 µg
    Specification mouse muscle cells
    Treatment transfected with GFP
    Gel Running Conditions Non-reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 31 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"