Overview

  • Product name
    Anti-Cyclophilin A antibody
    See all Cyclophilin A primary antibodies
  • Description
    Goat polyclonal to Cyclophilin A
  • Specificity
    This antibody is expected to recognise both isoform 1 (NP_066953.1) and isoform 2 (NP_982254.1 and NP_982255.1). This product is expected to recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to isoform 1 (NP_066953.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Dog, Pig, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla, Chinese hamster, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide:

    ERFGSRNGKTSKK

    from an internal region of the human Cyclophilin A protein sequence according to NP_066953.1; NP_982254.1; NP_982255.1.

  • Positive control
    • Human lymph node, tonsil and thymus lysates. Lysates prepared from transfected HEK293 cells transiently expressing Cyclophilin A.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

Target

Images

  • Anti-Cyclophilin A antibody (ab77188) at 0.1 µg/ml + Human Lymph node lysate (RIPA buffer) at 35 µg

    Predicted band size : 18 kDa
    Observed band size : 18 kDa

References

ab77188 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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