Anti-Cyclophilin F antibody (ab64935)

Overview

  • Product nameAnti-Cyclophilin F antibody
    See all Cyclophilin F primary antibodies
  • Description
    Rabbit polyclonal to Cyclophilin F
  • Specificityab64935 detects endogenous levels of total Cyclophilin F protein.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence within Human Cyclophilin F.

  • Positive control
    • Human brain tissue and extracts from HeLa cells, 293 cells and HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab64935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/20000.

Target

  • FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similaritiesBelongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • FormThis gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein.
  • Alternative names
    • Cyclophilin 3 antibody
    • cyclophilin D antibody
    • Cyclophilin F antibody
    • Cyp D antibody
    • CyP M antibody
    • CYP3 antibody
    • CypD antibody
    • hCyP3 antibody
    • mitochondrial antibody
    • Mitochondrial cyclophilin antibody
    • Peptidyl prolyl cis trans isomerase, mitochondral antibody
    • Peptidyl-prolyl cis-trans isomerase F antibody
    • Peptidyl-prolyl cis-trans isomerase F, mitochondrial antibody
    • Peptidylprolyl isomerase F (cyclophilin F) antibody
    • Peptidylprolyl isomerase F antibody
    • PPIase antibody
    • PPIase F antibody
    • Ppif antibody
    • PPIF_HUMAN antibody
    • Rotamase antibody
    • Rotamase F antibody
    see all

Anti-Cyclophilin F antibody images

  • ab64935, at a 1/50 dilution, staining Human Cyclophilin F in paraffin-embedded brain tissue, by Immunohistochemistry, with (+) or without (-) immunising peptide.
  • All lanes : Anti-Cyclophilin F antibody (ab64935) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from HepG2 cells
    Lane 4 : Extracts from HepG2 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • ICC/IF image of ab64935 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64935, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Cyclophilin F antibody (ab64935)

ab64935 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Spermophilus tridecemlineatus Tissue lysate - other (Liver whole, cytosol, mitochondria)
Gel Running Conditions Reduced Denaturing (12.5%)
Loading amount 20 µg
Specification Liver whole, cytosol, mitochondria
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Mr. James Bjork

Verified customer

Submitted Sep 25 2015


Sachez que ces anticorps sont contre la cyclophiline F ', PPIF, CYP3, SwissProt: P30405. Toutefois, dans le domaine mitochondrial, cette protéine est généralement appelé CYPD ou cyclophiline D - une protéine de 25 kDa dans les mitochondries.
...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Loading amount 20 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (15)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 07 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (mitochondria from HCT116 cells)
Total protein in input 500 µg
Specification mitochondria from HCT116 cells
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Sep 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"