For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Recombinant full length protein corresponding to Rat Cyclophilin F aa 1-206. (also known as CypD)
Our Abpromise guarantee covers the use of ab110324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|In-Cell ELISA||Use a concentration of 4 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Cyclophilin F knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Hek293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab110324 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab110324 was shown to specifically react with Cyclophilin F when Cyclophilin F knockout samples were used. Wild-type and Cyclophilin F knockout samples were subjected to SDS-PAGE. Ab110324 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This image is courtesy of an anonymous Abreview
Blocked with 5% milk for 1 hour at 25°C.
Incubated with the primary antibody diluted in PBS-T + 5% milk for 16 hours at 4°C.
Immunocytochemistry/ Immunofluorescence analysis of HEK293 cells labeling Cyclophilin F with ab110324 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with 1% triton x-100. 10% goat serum was used to blocke the cells for 1 hour at room temp followed by incubation with Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) in 10% goat serum-PBST for 16 hours at 4°C. A goat anti-mouse IgG secondary antibody was used at 1/300 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"