![Western blot - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) image](#Cyclophilin-40-Primary-antibodies-ab110324-6.jpg)
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) image](#Cyclophilin-40-Primary-antibodies-ab110324-7.gif)
![Flow Cytometry - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) image](#Cyclophilin-40-Primary-antibodies-ab110324-3.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Slides
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Cyclophilin F antibody [E11AE12BD4]
See all Cyclophilin F products (7) ...
Description
Mouse monoclonal [E11AE12BD4] to Cyclophilin F
Tested applications
WB, Flow Cyt, In-Cell ELISA, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Recombinant Rat Cyclophilin F.
Positive control
Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, HepG2 cells; Cultured Human embryonic lung-derived fibroblasts (strain MRC5); Human cerebellum tissue; HL60 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purification notes
ab110324 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
E11AE12BD4
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Mitochondrial
Immunology >> Immune Interventions >> Transplantation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.
Flow Cyt: Use a concentration of 1 µg/ml.
In-Cell ELISA: Use a concentration of 4 µg/ml.
ICC/IF: Use a concentration of 5 µg/ml.
IHC-P: 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Target
Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Sequence similarities
Belongs to the cyclophilin-type PPIase family.
Contains 1 PPIase cyclophilin-type domain.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP30405
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- Cyclophilin 3 antibody
- Cyclophilin D antibody
see all
Database links
- Entrez Gene: 414346 Cow
- Entrez Gene: 10105 Human
- Entrez Gene: 105675 Mouse
- Entrez Gene: 282819 Rat
- Omim: 604486 Human
- SwissProt: P30404 Cow
- SwissProt: P30405 Human
- SwissProt: Q99KR7 Mouse
see all
Anti-Cyclophilin F antibody [E11AE12BD4] images:
Western blot - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)
![Western blot - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)](/ps/datasheet/images/110/ab110324/Cyclophilin-40-Primary-antibodies-ab110324-6.jpg)
All lanes : Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Lane 5 : Isolated mitochondria from HepG2 cells at 20 µg
Predicted band size : 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)](/ps/datasheet/images/110/ab110324/Cyclophilin-40-Primary-antibodies-ab110324-7.gif)
Immunocytochemistry analysis using ab110324 at 1µg/ml staining Cyclophilin 40 in Cultured Human embryonic lung-derived fibroblasts (strain MRC5), (fixed, treated for heat-induced antigen retrieval, permeabilized) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
Flow Cytometry - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)
![Flow Cytometry - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)](/ps/datasheet/images/110/ab110324/Cyclophilin-40-Primary-antibodies-ab110324-3.jpg)
Flow cytometric analysis using ab110324 at 1µg/ml staining Cyclophilin F in HL60 cells (blue). Isotype control antibody (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)](/ps/datasheet/images/110/ab110324/Cyclophilin-40-Primary-antibodies-ab110324-8.gif)
Immunohistological analysis using ab110324 at 1µg/ml staining Cyclophilin F in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
Note: immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
References for Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)
This product has been referenced in:
- Levanets O et al. Mitochondrial DNA replication and OXPHOS gene transcription show varied responsiveness to Rieske protein knockdown in 143B cells. Biochimie 93:758-65 (2011).Read more (PubMed: 21251951) »
- Barrientos SA et al. Axonal degeneration is mediated by the mitochondrial permeability transition pore. J Neurosci 31:966-78 (2011).Read more (PubMed: 21248121) »
See all 24 publications for this product
Publishing research using ab110324? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"