Anti-Cyclosporin A antibody [CSZ.22] (ab8312)

Overview

  • Product nameAnti-Cyclosporin A antibody [CSZ.22]
  • Description
    Mouse monoclonal [CSZ.22] to Cyclosporin A
  • SpecificityThis antibody recognises Cyclosporin A in buffer solutions and whole blood extracts with Kd = 10-8
  • Tested applicationsSuitable for: ELISAmore details
  • General notes


    This antibody recognises Cyclosporin A

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody recognises Cyclosporin A
  • ClonalityMonoclonal
  • Clone numberCSZ.22
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1500.

Use at a dilution of 1/1500 for 2 hrs.

Target

  • RelevanceCyclosporin is used to prevent rejection following solid organ and bone marrow transplantation . It has been estimated that cyclosporine is given to more than 90% of children who have received a kidney transplant in the United States. In addition, the ability of cyclosporin to inhibit T-cell activation has been shown to have a role in the treatment of diseases such as nephrotic syndrome, refractory Crohn's disease and ulcerative colitis, biliary cirrhosis, aplastic anemia, rheumatoid arthritis, myasthenia gravis, and dermatomyositis.
  • Cellular localizationCytoplasmic

Anti-Cyclosporin A antibody [CSZ.22] images

  • ab8312 staining human PBMC, huh-7 cells by ELISA.  Cells were incubated in their respective growth medium with 10% fetal calf serum. At the end of the incubation they were washed with PBS (4°C) and the pellet was used to determine the CsA content. 

    1st step: cyclophilin B (ab53870, 1/1500 carbonate pH 8.5) was bound to the plate (overnight 4°C), 2nd step blocking agent, 3rd step incubation with cell lysate incubated with CsA or analogs (CsA-OH) overnight,
    4th step ab8312 mouse anti CsA, 1/500 for 2 hours at 37°C
    5th step ab6729 (1/2000) Alkalline phosphatase reading at 405 nm

    See Abreview

References for Anti-Cyclosporin A antibody [CSZ.22] (ab8312)

ab8312 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your reply.

When using samples like blood that contain endogenous IgG's, I recommend using a pre-adsorbed secondary antibody that won't bind to the IgG molecules in your samples. Using ab8312 as the primary antibody, I...

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Thank you for your call today and for your patience while I've been in touch with the production lab regarding this antibody.

As we discussed, ab8312 has only been tested in indirect ELISA by coating the plate with antigen and adding the anti...

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Thank you very much for contacting us with your question.

The cyclosporin A antibody, ab8312, is suitable for detecting synthesized cyclosporin in rat samples, however it has only been tested in ELISA at this time. What kind of assay would yo...

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Thank you very much for your interest in ab8312.

To our knowledge, ab8312 has not been tested inICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab8312 now, test it in ICC/IFand submit feedback to us in the form of ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (PBMC, huh-7 cells)
Specification PBMC, huh-7 cells
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Type Sandwich (Detection)
Username

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Submitted Jun 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"