Overview

  • Product name
  • Description
    Rabbit polyclonal to CYFIP1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Lizard, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1203 and 1253 of Human CYFIP1 (NP_055423.1).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab108220 was affinity purified using an epitope specific to CYFIP1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab108220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 145 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.
  • Sequence similarities
    Belongs to the CYFIP family.
  • Cellular localization
    Cytoplasm > perinuclear region. Cell projection > lamellipodium. Cell projection > ruffle. Cell junction > synapse > synaptosome. Highly expressed in the perinuclear region. Enriched in synaptosomes, membrane ruffles and at the tips of lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • CY antibody
    • CYFIP 1 antibody
    • CYFIP1 antibody
    • CYFP1_HUMAN antibody
    • Cytoplasmic FMR1 interacting protein 1 antibody
    • Cytoplasmic FMR1-interacting protein 1 antibody
    • FLJ45151 antibody
    • KIAA0068 antibody
    • p140sra 1 antibody
    • p140sra-1 antibody
    • P140SRA1 antibody
    • Selective hybridizing clone antibody
    • SHYC antibody
    • Specifically Rac1 associated protein 1 antibody
    • Specifically Rac1-associated protein 1 antibody
    • SRA 1 antibody
    • Sra-1 antibody
    • SRA1 antibody
    see all

Images

  • All lanes : Anti-CYFIP1 antibody (ab108220) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 145 kDa


    Exposure time : 3 minutes
  • ab108220 at 1 µg/ml detecting CYFIP1 in HeLa whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes an upstream epitope of CYFIP1
    Lane 2: ab108220 at 6 µg/mg of lysate
    Lane 3: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 30 seconds

References

This product has been referenced in:
  • Han K  et al. Fragile X-like behaviors and abnormal cortical dendritic spines in cytoplasmic FMR1-interacting protein 2-mutant mice. Hum Mol Genet 24:1813-23 (2015). Read more (PubMed: 25432536) »

See 1 Publication for this product

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