Overview

  • Product name
  • Description
    Rabbit polyclonal to CYFIP2
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CYFIP2.

    (Peptide available as ab100822.)

  • Positive control
    • This antibody gave a positive signal in Jurkat Nuclear Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab79716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24446484
WB 1/250. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).

Target

  • Relevance
    CYFIP2 is involved in T cell adhesion and p53 dependent induction of apoptosis. It does not bind RNA but is up regulated significantly in CD4+ T lymphocytes from patients with multiple sclerosis. There are 2 isoforms produced by alternative splicing.
  • Cellular localization
    Cytoplasm perinuclear region. Cell junction, synapse, synaptosome. Treatment with leptomycin-B triggers translocation to the nucleus.
  • Database links
  • Alternative names
    • CYFP2 antibody
    • cytoplasmic FMR1 interacting protein 2 antibody
    • KIAA1168 antibody
    • p53 inducible protein antibody
    • p53-inducible protein 121 antibody
    • PIR121 antibody
    see all

Images

  • Anti-CYFIP2 antibody (ab79716) at 1/250 dilution + Jurkat nuclear extract lysate (ab14844) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 148 kDa
    Observed band size : 148 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

References

This product has been referenced in:
  • Toret CP  et al. A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion. J Cell Biol 204:265-79 (2014). ICC/IF, WB ; Drosophila melanogaster . Read more (PubMed: 24446484) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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