The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 24446484
1/250. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).
RelevanceCYFIP2 is involved in T cell adhesion and p53 dependent induction of apoptosis. It does not bind RNA but is up regulated significantly in CD4+ T lymphocytes from patients with multiple sclerosis. There are 2 isoforms produced by alternative splicing.
Cellular localizationCytoplasm perinuclear region. Cell junction, synapse, synaptosome. Treatment with leptomycin-B triggers translocation to the nucleus.