Overview

  • Product nameCyGEL™
  • Tested applicationsSuitable for: FM, Functional Studies, ICC/IFmore details
  • General notesCyGEL™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling.
    CyGEL™ has many applications in imaging and cell-based screening.
    Key features of CyGEL™ include:
    1. Convenient immobilization of non-adherent cells.
    2. Controllable and rapid transition from liquid to gel, which allows quick preparation of live cells for gel mounting.
    3. Optically clear with low autofluorescence.
    4. Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).

    CyGEL™ is recommended for mounting live de-adherent/ non-adherent cells for microscopy, or mounting live C. elegans and parasites (e.g. leishmania, trypanosomes), which can be recovered afterwards. CyGEL™ is provided as concentrated non-buffered form. Add the concentrated PBS supplement (provided) to prime CyGEL™ prior use.
    CyGEL™ is supplied as 8 vials, each containing 500µl, and 1 vial of 40xPBS.
    For imaging experiments longer than 2 hours, we recommend using CyGEL Sustain™ (ab109205).

Properties

    Applications

    Our Abpromise guarantee covers the use of ab109204 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    FM Use at an assay dependent concentration.
    Functional Studies Use at an assay dependent dilution.
    ICC/IF Use at an assay dependent dilution.
    Please refer to the Protocol Booklet for detailed information about the use of CyGEL.

    CyGEL™ images

    • This ilustration demonstrates the general procedure for using CyGEL™ with live cells prior analysis by fluorescence microscopy.

    Protocols

    References for CyGEL™ (ab109204)

    ab109204 has not yet been referenced specifically in any publications.

    Product Wall

    excellent method

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    This is a good method to perform single cell analysis and afterwards to cultivate the cells again or to make additional testings.
    It works well and reliably.
    The instructions are easy to understand and easy to implement.
    We have used it for immunofluorescence analysis.
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    Verified customer

    Submitted Oct 07 2015

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    As suggested, there is no particular reason to use CyGEL Sustain over CyGEL (a PBS-based formulation) other than the ease of adding specific media.

    We have limited data on the long-term ima...

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    Thank you for contacting us.

    I am sorry you are not able to enter our Website, please do let us know if the problem persists. You will find attached the protocols for our CyGel products: ab109205 and ab109204.

    These products should ...

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    CyGEL is a Michelle structure and like most gels will not permit passive diffusion of macromolecules such and large proteins.

    However, if the intention is to label cells with the tagged antibody in suspension and then wash and add them to CyG...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"