For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
CyGEL™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling.
CyGEL™ has many applications in imaging and cell-based screening.
Key features of CyGEL™ include:
CyGEL™ is recommended for mounting live de-adherent/ non-adherent cells for microscopy, or mounting live C. elegans and parasites (e.g. leishmania, trypanosomes), which can be recovered afterwards. CyGEL™ is provided as concentrated non-buffered form. Add the concentrated PBS supplement (provided) to prime CyGEL™ prior use.
CyGEL™ is supplied as 8 vials, each containing 500µl, and 1 vial of 40xPBS.
For imaging experiments longer than 2 hours, we recommend using CyGEL Sustain™ (ab109205).
Our Abpromise guarantee covers the use of ab109204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.
Please refer to the Protocol Booklet for detailed information about the use of CyGEL.
ab109204 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"