For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
CyGEL Sustain™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL Sustain™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling. CyGEL Sustain™ has many applications in imaging and cell-based screening.
Key features of CyGEL Sustain™ include:
CyGEL Sustain™ is recommended for mounting live de-adherent/ non-adherent cells and Zebrafish embryos for microscopy. CyGEL Sustain™ is specially formulated to allow addition of RPMI and similar culture media, which makes it the best tool for extended imaging experiments, as cells will survive for several hours.
CyGEL Sustain™ is supplied as 10 vials, each containing 500µl.
For live C. elegans and parasites we recommend CyGEL™(ab109204).
Our Abpromise guarantee covers the use of ab109205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.
Please refer to the Protocol Booklet for detailed information about the use of CyGEL Sustain.
|FM||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent dilution.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"