Overview

  • Product name
    Anti-CYLC2 antibody
  • Description
    Rabbit polyclonal to CYLC2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 300-349 (DAKKVAKKDT EKESADSKKD AKKNAKKDAK KDAKKNAKKD EKKDAKKKGK) of Human CYLC2 (NP_001331).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • Relevance
    CYCL2 (Cylicin II) is specifically expressed in testis and is part of the cytoskeletal calyx of mammalian sperm heads. Cylicin II may play a role in the morphogenesis of the sperm head and may be involved in spermatid differentiation.
  • Cellular localization
    Cytoplasm, cytoskeleton, perinuclear theca, calyx. Note=Sperm head cytoskeletal structure.
  • Database links
  • Alternative names
    • CYL2 antibody
    • CYLC 2 antibody
    • Cylicin 2 antibody
    • Cylicin basic protein of sperm head cytoskeleton 2 antibody
    • Cylicin II antibody
    • Cylicin2 antibody
    • MGC129591 antibody
    • Multiple band polypeptide II antibody
    see all

Images

  • Anti-CYLC2 antibody (ab81228) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,50 kDa. We are unsure as to the identity of these extra bands.

References

ab81228 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab81228.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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