• Product nameAnti-CYP1B1 antibody
    See all CYP1B1 primary antibodies
  • Description
    Rabbit polyclonal to CYP1B1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CYP1B1.

    (Peptide available as ab33584.)

  • Positive control
    • Ab32649 gave a positive signal in the following human tissue lysates: Brain; Kidney; Liver. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: HeLa.


Associated products


Our Abpromise guarantee covers the use of ab32649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
IHC-P 1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
    Participates in the metabolism of an as-yet-unknown biologically active molecule that is a participant in eye development.
  • Tissue specificityExpressed in many tissues.
  • Involvement in diseaseDefects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor.
    Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG) [MIM:137760]. POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes.
    Defects in CYP1B1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly is a congenital defect of the anterior chamber of the eye.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aryl hydrocarbon hydroxylase antibody
    • CP1B antibody
    • CP1B1_HUMAN antibody
    • Cyp1b1 antibody
    • CYPIB1 antibody
    • Cytochrome P450 1B1 antibody
    • Cytochrome P450 family 1 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody
    • Flavoprotein linked monooxygenase antibody
    • GLC3A antibody
    • Microsomal monooxygenase antibody
    • P4501B1 antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-CYP1B1 antibody images

  • All lanes : Anti-CYP1B1 antibody (ab32649) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue
    Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Image courtesy of Human Protein Atlas

    ab32649 staining CYP1B1 in human stomach. Paraffin embedded human stomach tissue was incubated with ab32649 (1/1500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab32649 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab32649 stained HeLa cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32649 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CYP1B1 antibody (ab32649)

This product has been referenced in:
  • Hevir N  et al. Disturbed balance between phase I and II metabolizing enzymes in ovarian endometriosis: A source of excessive hydroxy-estrogens and ROS? Mol Cell Endocrinol : (2012). WB, IHC-P ; Human . Read more (PubMed: 23277161) »
  • Hevir N  et al. Disturbed expression of phase I and phase II estrogen-metabolizing enzymes in endometrial cancer: lower levels of CYP1B1 and increased expression of S-COMT. Mol Cell Endocrinol 331:158-67 (2011). WB, IHC-P ; Human . Read more (PubMed: 20887769) »

See all 2 Publications for this product

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Thank you for contacting Abcam. I am sorry that you are having problems with ab32649 in western blotting. I have a few more questions/suggestions about the protocol that you are using which could help. 1 - Are you seeing any bands at 70kDa, which could...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
Loading amount 60 µg
Specification endometrial cancer and adjacent control endometriu
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (endometrial cancer tissue)
Specification endometrial cancer tissue
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate buffer in microwave oven
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 08 2011