• Product nameAnti-CYP1B1 antibody
    See all CYP1B1 primary antibodies
  • Description
    Rabbit polyclonal to CYP1B1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Dog, Chimpanzee, Monkey, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a portion of amino acids 200-250 of human CYP1B1.

  • Positive control
    • Testis tissue



Our Abpromise guarantee covers the use of ab78044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24497629
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 61 kDa.
IHC-P Use a concentration of 10 µg/ml.


  • FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
    Participates in the metabolism of an as-yet-unknown biologically active molecule that is a participant in eye development.
  • Tissue specificityExpressed in many tissues.
  • Involvement in diseaseDefects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor.
    Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG) [MIM:137760]. POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes.
    Defects in CYP1B1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly is a congenital defect of the anterior chamber of the eye.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aryl hydrocarbon hydroxylase antibody
    • CP1B antibody
    • CP1B1_HUMAN antibody
    • Cyp1b1 antibody
    • CYPIB1 antibody
    • Cytochrome P450 1B1 antibody
    • Cytochrome P450 family 1 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody
    • Flavoprotein linked monooxygenase antibody
    • GLC3A antibody
    • Microsomal monooxygenase antibody
    • P4501B1 antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-CYP1B1 antibody images

  • ab78044, at a concentration of 10 µ/ml, staining CYP1B1 in formalin fixed, paraffin embedded testis tissue by Immunohistochemistry.
  • ab78044 staining CYP1B1 in Mouse heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab78044 staining CYP1B1 in Mouse testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • All lanes : Anti-CYP1B1 antibody (ab78044) at 0.5 µg/ml

    Lane 1 : Human brain
    Lane 2 : Human brain with immunizing peptide

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : ~100 kDa,~120 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemistry/Immunofluorescence analysis of COS-7 cells (grown on coverslips were transfected with vector alone or wild wtype (WT) CYP1B1 cDNAs) labelling CYP1B1 with ab78044 at 1/100. The cells were fixed with chilled methanol, and cells were also immunostained with a mouse anti-CcOI (1/100). The cells were then stained with Alexa 488-conjugated anti-rabbit antibody and Alexa 594-conjugated anti-mouse IgG (both 1/100). The stained patterns were overlaid.

  • All lanes : Anti-CYP1B1 antibody (ab78044) at 1/500 dilution

    Lane 1 : Tissue lysate prepared from rat brain capillary membranes, treated with complete protease inhibitor
    Lane 2 : Tissue lysate prepared from rat brain, treated with complete protease inhibitor
    Lane 3 : Tissue lysate prepared from rat choroid plexus, treated with complete protease inhibitor
    Lane 4 : Tissue lysate prepared from rat kidney, treated with complete protease inhibitor
    Lane 5 : Tissue lysate prepared from rat liver, treated with complete protease inhibitor

    Lysates/proteins at 10 µg per lane.

    HRP conjugated goat anti-rabbit IgG (H+L) at 1/15000 dilution

    Predicted band size : 61 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-CYP1B1 antibody (ab78044)

This product has been referenced in:
  • Bansal S  et al. Mitochondrial targeting of cytochrome P450 (CYP) 1B1 and its role in polycyclic aromatic hydrocarbon-induced mitochondrial dysfunction. J Biol Chem 289:9936-51 (2014). Electron Microscopy, ICC/IF ; Human . Read more (PubMed: 24497629) »
  • Chan JK  et al. Combustion derived ultrafine particles induce cytochrome P-450 expression in specific lung compartments in the developing neonatal and adult rat. Am J Physiol Lung Cell Mol Physiol 304:L665-77 (2013). Rat . Read more (PubMed: 23502512) »

See all 4 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (brain capillary membranes, brain, choroid plexus,)
Loading amount 2 µg
Specification brain capillary membranes, brain, choroid plexus,
Treatment Roche Diagnostics Complete Protease Inhibitor
Gel Running Conditions Reduced Denaturing (Invitrogen NuPage Bis-Tris 4-12%, 1 mm x 15 wells)
Blocking step Pierce Protein-free T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

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Verified customer

Submitted Mar 05 2012

Thank you for your inquiry and I apologize for the delay in this reply.  I had the lab confirm that they have tested this antibody in mouse in IHC, but not in WB.  Though they have tested it in human brain lysate (see attached).  Lane 2 was generat...

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Thank you for your enquiry, The volume can be worked out from the concentration and weight per vial provided on the datasheet.   50 µg provided at 0.5 mg/ml Concentration is 500 ug in 1000 ul Then for  50 ug:  500ug / 50ug is a ratio of 1:...

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