Anti-CYP21A2 antibody (ab64887)
Key features and details
- Rabbit polyclonal to CYP21A2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYP21A2 antibody
See all CYP21A2 primary antibodies -
Description
Rabbit polyclonal to CYP21A2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from an internal sequence of human CYP21A2.
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Positive control
- extracts from 293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/100. Detects a band of approximately 56, 40 kDa (predicted molecular weight: 56 kDa).
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Notes |
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WB
1/500 - 1/100. Detects a band of approximately 56, 40 kDa (predicted molecular weight: 56 kDa). |
Target
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Function
Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids. -
Involvement in disease
Adrenal hyperplasia 3 -
Sequence similarities
Belongs to the cytochrome P450 family. -
Domain
The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1589 Human
- Omim: 613815 Human
- SwissProt: P08686 Human
- Unigene: 654479 Human
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Alternative names
- CYP21 antibody
- 21 OHase antibody
- 21-OHase antibody
see all
Images
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All lanes : Anti-CYP21A2 antibody (ab64887) at 1/500 dilution
Lane 1 : extracts from 293 cells (5-30µg total protein)
Lane 2 : extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab64887 has been referenced in 2 publications.
- Bouhaddioui W et al. CYP21A2 expression is localized in the developing distal epithelium of the human perinatal lung and is compatible with in situ production and intracrine actions of active glucocorticoids. J Steroid Biochem Mol Biol 163:12-9 (2016). PubMed: 27004467
- Idkowiak J et al. Concomitant mutations in the P450 oxidoreductase and androgen receptor genes presenting with 46,XY disordered sex development and androgenization at adrenarche. J Clin Endocrinol Metab 95:3418-27 (2010). WB ; Human . PubMed: 20410220