Overview

  • Product name
  • Description
    Rabbit polyclonal to CYP21A2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human CYP21A2.

  • Positive control
    • extracts from 293 cells.

Applications

Our Abpromise guarantee covers the use of ab64887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/100. Detects a band of approximately 56, 40 kDa (predicted molecular weight: 56 kDa).

Target

  • Function
    Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.
  • Involvement in disease
    Adrenal hyperplasia 3
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Domain
    The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYP21 antibody
    • 21 OHase antibody
    • 21-OHase antibody
    • CA21H antibody
    • CAH1 antibody
    • CP21A_HUMAN antibody
    • CPS1 antibody
    • CYP21A 2 antibody
    • CYP21A2 antibody
    • CYP21B antibody
    • Cytochrome P-450c21 antibody
    • Cytochrome P450 21 antibody
    • Cytochrome P450 C21B antibody
    • Cytochrome P450 XXI antibody
    • Cytochrome P450, family 21, subfamily A, polypeptide 2 antibody
    • Cytochrome P450, subfamily XXIA (steroid 21 hydroxylase, congenital adrenal hyperplasia), polypeptide 2 antibody
    • Cytochrome P450-C21 antibody
    • Cytochrome P450-C21B antibody
    • P450 C21 antibody
    • P450 C21B antibody
    • P450c21B antibody
    • Steroid 21 hydroxylase antibody
    • Steroid 21 monooxygenase antibody
    • Steroid 21-hydroxylase antibody
    see all

Images

  • All lanes : Anti-CYP21A2 antibody (ab64887) at 1/500 dilution

    Lane 1 : extracts from 293 cells (5-30µg total protein)
    Lane 2 : extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Bouhaddioui W  et al. CYP21A2 expression is localized in the developing distal epithelium of the human perinatal lung and is compatible with in situ production and intracrine actions of active glucocorticoids. J Steroid Biochem Mol Biol 163:12-9 (2016). Read more (PubMed: 27004467) »
  • Idkowiak J  et al. Concomitant mutations in the P450 oxidoreductase and androgen receptor genes presenting with 46,XY disordered sex development and androgenization at adrenarche. J Clin Endocrinol Metab 95:3418-27 (2010). WB ; Human . Read more (PubMed: 20410220) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. The immunogen sequence is from 150aa to 200aa. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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