Overview

  • Product nameAnti-CYP24A1 antibody
    See all CYP24A1 primary antibodies
  • Description
    Mouse monoclonal to CYP24A1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Human
  • Immunogen

    Recombinant fragment: LMLNTQVLGS SEDNFEDSSQ FRPERWLQEK EKINPFAHLP FGVGKRMCIG RRLAELQLHL ALCWIVRKYD IQATDNEPVE MLHSGTLVPS RELPIAFCQR , corresponding to amino acids 415-515 of Human CYP24A1

Applications

Our Abpromise guarantee covers the use of ab54594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • FunctionHas a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 25 @dihydroxyvitamin D3 24 hydroxylase antibody
    • 1 25 dihydroxyvitamin D(3) 24 hydroxylase antibody
    • 1 antibody
    • 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial antibody
    • 24 OHase antibody
    • 24-OHase antibody
    • 25-dihydroxyvitamin D(3) 24-hydroxylase antibody
    • CP 24 antibody
    • CP24 antibody
    • CP24A_HUMAN antibody
    • CYP 24 antibody
    • CYP24 antibody
    • CYP24A1 antibody
    • Cytochrome P450 24A1 antibody
    • Cytochrome P450 24A1 mitochondrial antibody
    • cytochrome P450 CC24 antibody
    • Cytochrome P450 family 24 antibody
    • Cytochrome P450 family 24 subfamily A polypeptide 1 antibody
    • Cytochrome P450 subfamily XXIV antibody
    • Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase) antibody
    • Cytochrome P450-CC24 antibody
    • EC 1.14.13.n4 antibody
    • Exo mitochondrial protein antibody
    • HCAI antibody
    • MGC126273 antibody
    • MGC126274 antibody
    • mitochondrial antibody
    • P450 CC24 antibody
    • Vitamin D 24 hydroxylase antibody
    • Vitamin D(3) 24 hydroxylase antibody
    • Vitamin D(3) 24-hydroxylase antibody
    see all

Anti-CYP24A1 antibody images

  • Western blot against tagged recombinant protein immunogen using ab54594 CYP24A1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

References for Anti-CYP24A1 antibody (ab54594)

ab54594 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (vascular smooth muscle cell)
Specification vascular smooth muscle cell
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Mar 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"