Overview

  • Product nameAnti-CYP24A1 antibody
    See all CYP24A1 primary antibodies
  • Description
    Rabbit polyclonal to CYP24A1
  • SpecificityDetects endogenous levels of total CYP24A1 protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from internal sequence of human CYP24A1.

  • Positive control
    • Extracts from HUVEC cells

Properties

Applications

Our Abpromise guarantee covers the use of ab65956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/10000.
    WB: 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 59 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHas a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.
    • Sequence similaritiesBelongs to the cytochrome P450 family.
    • Cellular localizationMitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 25 @dihydroxyvitamin D3 24 hydroxylase antibody
      • 1 25 dihydroxyvitamin D(3) 24 hydroxylase antibody
      • 1 antibody
      • 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial antibody
      • 24 OHase antibody
      • 24-OHase antibody
      • 25-dihydroxyvitamin D(3) 24-hydroxylase antibody
      • CP 24 antibody
      • CP24 antibody
      • CP24A_HUMAN antibody
      • CYP 24 antibody
      • CYP24 antibody
      • CYP24A1 antibody
      • Cytochrome P450 24A1 antibody
      • Cytochrome P450 24A1 mitochondrial antibody
      • cytochrome P450 CC24 antibody
      • Cytochrome P450 family 24 antibody
      • Cytochrome P450 family 24 subfamily A polypeptide 1 antibody
      • Cytochrome P450 subfamily XXIV antibody
      • Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase) antibody
      • Cytochrome P450-CC24 antibody
      • EC 1.14.13.n4 antibody
      • Exo mitochondrial protein antibody
      • HCAI antibody
      • MGC126273 antibody
      • MGC126274 antibody
      • mitochondrial antibody
      • P450 CC24 antibody
      • Vitamin D 24 hydroxylase antibody
      • Vitamin D(3) 24 hydroxylase antibody
      • Vitamin D(3) 24-hydroxylase antibody
      see all

    Anti-CYP24A1 antibody images

    • All lanes : Anti-CYP24A1 antibody (ab65956) at 1/500 dilution

      Lane 1 : extracts from HUVEC cells
      Lane 2 : extracts from HUVEC cells with immunizing peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 59 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-CYP24A1 antibody (ab65956)

    ab65956 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab65956.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"