The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 59 kDa.
Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue, labeling CYP24A1 with ab175976 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Schüler-Toprak S et al. Agonists and knockdown of estrogen receptor ß differentially affect invasion of triple-negative breast cancer cells in vitro. BMC Cancer16:951 (2016).
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