Overview

  • Product name
    Anti-CYP24A1 antibody - C-terminal
    See all CYP24A1 primary antibodies
  • Description
    Rabbit polyclonal to CYP24A1 - C-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CYP24A1 aa 473-502 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q07973

  • Positive control
    • Mouse spleen tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab175976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 59 kDa.

Target

  • Function
    Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 25 @dihydroxyvitamin D3 24 hydroxylase antibody
    • 1 25 dihydroxyvitamin D(3) 24 hydroxylase antibody
    • 1 antibody
    • 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial antibody
    • 24 OHase antibody
    • 24-OHase antibody
    • 25-dihydroxyvitamin D(3) 24-hydroxylase antibody
    • CP 24 antibody
    • CP24 antibody
    • CP24A_HUMAN antibody
    • CYP 24 antibody
    • CYP24 antibody
    • CYP24A1 antibody
    • Cytochrome P450 24A1 antibody
    • Cytochrome P450 24A1 mitochondrial antibody
    • cytochrome P450 CC24 antibody
    • Cytochrome P450 family 24 antibody
    • Cytochrome P450 family 24 subfamily A member 1 antibody
    • Cytochrome P450 family 24 subfamily A polypeptide 1 antibody
    • Cytochrome P450 subfamily XXIV antibody
    • Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase) antibody
    • Cytochrome P450-CC24 antibody
    • EC 1.14.13.n4 antibody
    • Exo mitochondrial protein antibody
    • HCAI antibody
    • HCINF1 antibody
    • MGC126273 antibody
    • MGC126274 antibody
    • mitochondrial antibody
    • P450 CC24 antibody
    • Vitamin D 24 hydroxylase antibody
    • Vitamin D(3) 24 hydroxylase antibody
    • Vitamin D(3) 24-hydroxylase antibody
    see all

Images

  • Anti-CYP24A1 antibody - C-terminal (ab175976) at 1/1000 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size : 59 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue, labeling CYP24A1 with ab175976 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

This product has been referenced in:
  • Schüler-Toprak S  et al. Agonists and knockdown of estrogen receptor ß differentially affect invasion of triple-negative breast cancer cells in vitro. BMC Cancer 16:951 (2016). Read more (PubMed: 28003019) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Vascular smooth muscle cell
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Oct 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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