Recombinant
RabMAb

Anti-CYP26A1 antibody [EPR11841] (ab172474)

Overview

  • Product name
    Anti-CYP26A1 antibody [EPR11841]
    See all CYP26A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11841] to CYP26A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human CYP26A1 aa 250-450.
    Database link: O43174

  • Positive control
    • Human placenta, fetal liver and fetal kidney tissue lysates; 293T and HepG2 cell lysates; Human liver and pancreas tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA.
    • Tissue specificity
      Highest levels in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain.
    • Sequence similarities
      Belongs to the cytochrome P450 family.
    • Cellular localization
      Endoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CP26 antibody
      • CP26A_HUMAN antibody
      • CYP26 antibody
      • CYP26A 1 antibody
      • cyp26a1 antibody
      • Cytochrome P450 26A1 antibody
      • Cytochrome P450 family 26 subfamily A polypeptide 1 antibody
      • Cytochrome P450 retinoic acid-inactivating 1 antibody
      • cytochrome P450, family 26, subfamily A, polypeptide 1 antibody
      • cytochrome P450, subfamily XXVIA, polypeptide 1 antibody
      • Cytochrome P450RAI antibody
      • hP450RAI antibody
      • OTTHUMP00000020102 antibody
      • OTTHUMP00000020103 antibody
      • P450 antibody
      • P450 retinoic acid inactivating 1 antibody
      • P450RAI antibody
      • P450RAI1 antibody
      • Retinoic acid 4 hydroxylase antibody
      • Retinoic acid 4-hydroxylase antibody
      • Retinoic acid metabolizing cytochrome antibody
      • Retinoic acid-metabolizing cytochrome antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CYP26A1 with ab172474 at 1/250 dilution.

    • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling CYP26A1 with ab172474 at 1/250 dilution.

    • ab172474 showing +ve staining in Human normal colon tissue.

    • ab172474 showing +ve staining in Human normal uterus tissue.

    • ab172474 showing -ve staining in Human skeletal muscle tissue.
    • ab172474 showing -ve staining in Human heart muscle tissue.
    • All lanes : Anti-CYP26A1 antibody [EPR11841] (ab172474) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Human fetal liver tissue lysate
      Lane 3 : Human fetal kidney tissue lysate
      Lane 4 : 293T cell lysate
      Lane 5 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 56 kDa

    References

    This product has been referenced in:
    • Huang YJ  et al. Protection against acetaminophen-induced acute liver failure by omentum adipose tissue derived stem cells through the mediation of Nrf2 and cytochrome P450 expression. J Biomed Sci 23:5 (2016). WB ; Mouse . Read more (PubMed: 26787241) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK17 cell line)
    Gel Running Conditions
    Reduced Denaturing (Gel 10%)
    Loading amount
    10 µg
    Specification
    BHK17 cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Simon Boudreault

    Verified customer

    Submitted Mar 23 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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