Overview

  • Product nameAnti-CYP27A1 antibody
    See all CYP27A1 primary antibodies
  • Description
    Rabbit polyclonal to CYP27A1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) from an internal sequence.

  • Positive control
    • Extracts from HeLa cells.

Applications

Our Abpromise guarantee covers the use of ab64889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

Target

  • FunctionCatalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
  • PathwayHormone biosynthesis; cholecalciferol biosynthesis.
  • Involvement in diseaseCerebrotendinous xanthomatosis
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationMitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12-alpha-triol 27-hydroxylase antibody
    • 5-beta-cholestane-3-alpha antibody
    • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
    • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody
    • 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody
    • 7-alpha antibody
    • Cholestanetriol 26 monooxygenase antibody
    • CP27 antibody
    • CP27A_HUMAN antibody
    • CTX antibody
    • CYP antibody
    • CYP27 antibody
    • CYP27A1 antibody
    • Cytochrome P 450C27/25 antibody
    • Cytochrome P-450C27/25 antibody
    • Cytochrome P450 27 antibody
    • Cytochrome P450 27 mitochondrial antibody
    • Cytochrome P450 family 27 subfamily A polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody
    • mitochondrial antibody
    • Sterol 26 hydroxylase antibody
    • Sterol 26 hydroxylase mitochondrial antibody
    • Sterol 26-hydroxylase antibody
    • Sterol 27 hydroxylase antibody
    • Sterol 27-hydroxylase antibody
    • Vitamin D(3) 25 hydroxylase antibody
    • Vitamin D(3) 25-hydroxylase antibody
    see all

Anti-CYP27A1 antibody images

  • All lanes : Anti-CYP27A1 antibody (ab64889) at 1/500 dilution

    Lane 1 : extracts from HeLa cells (5-30µg total protein)
    Lane 2 : extracts from HeLa cells (5-30µg total protein) and 5-10µg of the immunising peptide.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • ab64889 staining CYP27A1 in human colon tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 0.4% Casein in phosphate-buffered saline for 30 minutes at 25°C. Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 (TBST) for 1 hour at 25°C.  

    See Abreview

  • ICC/IF image of ab64889 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64889, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CYP27A1 antibody (ab64889)

This product has been referenced in:
  • Li J  et al. De novo synthesis of steroids and oxysterols in adipocytes. J Biol Chem 289:747-64 (2014). WB . Read more (PubMed: 24280213) »
  • Bergadà L  et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest 94:608-22 (2014). Read more (PubMed: 24732451) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO 1x Target Retrieval Solution
Permeabilization No
Blocking step Casein in phosphate-buffered saline as blocking agent for 30 minute(s) · Concentration: 0.4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"