Overview

  • Product nameAnti-CYP27A1 antibody
    See all CYP27A1 primary antibodies
  • Description
    Rabbit polyclonal to CYP27A1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 432-481 (SRDPTAFSEP ESFQPHRWLR NSQPATPRIQ HPFGSVPFGY GVRACLGRRI) of human CYP27A1 (NP_000775).

  • Positive control
    • Fetal brain lysate.

Applications

Our Abpromise guarantee covers the use of ab82926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

Target

  • FunctionCatalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
  • PathwayHormone biosynthesis; cholecalciferol biosynthesis.
  • Involvement in diseaseCerebrotendinous xanthomatosis
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationMitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12-alpha-triol 27-hydroxylase antibody
    • 5-beta-cholestane-3-alpha antibody
    • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
    • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody
    • 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody
    • 7-alpha antibody
    • Cholestanetriol 26 monooxygenase antibody
    • CP27 antibody
    • CP27A_HUMAN antibody
    • CTX antibody
    • CYP antibody
    • CYP27 antibody
    • CYP27A1 antibody
    • Cytochrome P 450C27/25 antibody
    • Cytochrome P-450C27/25 antibody
    • Cytochrome P450 27 antibody
    • Cytochrome P450 27 mitochondrial antibody
    • Cytochrome P450 family 27 subfamily A polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody
    • mitochondrial antibody
    • Sterol 26 hydroxylase antibody
    • Sterol 26 hydroxylase mitochondrial antibody
    • Sterol 26-hydroxylase antibody
    • Sterol 27 hydroxylase antibody
    • Sterol 27-hydroxylase antibody
    • Vitamin D(3) 25 hydroxylase antibody
    • Vitamin D(3) 25-hydroxylase antibody
    see all

Anti-CYP27A1 antibody images

  • Anti-CYP27A1 antibody (ab82926) at 1 µg/ml + human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-CYP27A1 antibody (ab82926)

ab82926 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"