Overview

  • Product nameAnti-CYP27A1 antibody
    See all CYP27A1 primary antibodies
  • Description
    Mouse polyclonal to CYP27A1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human CYP27A1 (NP_000775.1)

  • Positive control
    • CYP27A1 transfected 293T cell lysate and human liver tissue lysate.

Applications

Our Abpromise guarantee covers the use of ab89781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.

Target

  • FunctionCatalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
  • PathwayHormone biosynthesis; cholecalciferol biosynthesis.
  • Involvement in diseaseCerebrotendinous xanthomatosis
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationMitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12-alpha-triol 27-hydroxylase antibody
    • 5-beta-cholestane-3-alpha antibody
    • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
    • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody
    • 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody
    • 7-alpha antibody
    • Cholestanetriol 26 monooxygenase antibody
    • CP27 antibody
    • CP27A_HUMAN antibody
    • CTX antibody
    • CYP antibody
    • CYP27 antibody
    • CYP27A1 antibody
    • Cytochrome P 450C27/25 antibody
    • Cytochrome P-450C27/25 antibody
    • Cytochrome P450 27 antibody
    • Cytochrome P450 27 mitochondrial antibody
    • Cytochrome P450 family 27 subfamily A polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody
    • mitochondrial antibody
    • Sterol 26 hydroxylase antibody
    • Sterol 26 hydroxylase mitochondrial antibody
    • Sterol 26-hydroxylase antibody
    • Sterol 27 hydroxylase antibody
    • Sterol 27-hydroxylase antibody
    • Vitamin D(3) 25 hydroxylase antibody
    • Vitamin D(3) 25-hydroxylase antibody
    see all

Anti-CYP27A1 antibody images

  • Anti-CYP27A1 antibody (ab89781) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size : 60 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CYP27A1 antibody (ab89781) at 1 µg/ml

    Lane 1 : CYP27A1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-CYP27A1 antibody (ab89781)

ab89781 has not yet been referenced specifically in any publications.

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