Recombinant
RabMAb

Anti-CYP27A1 antibody [EPR7529] (ab126785)

Overview

  • Product name
    Anti-CYP27A1 antibody [EPR7529]
    See all CYP27A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7529] to CYP27A1
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human CYP27A1 (UniProt Q02318).

  • Positive control
    • WB: HepG2, Caco 2, human fetal liver and mouse liver lysates. IHC-P: Human kidney and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab126785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.Can be blocked with CYP27A1 peptide (ab139504).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/120.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
    • Pathway
      Hormone biosynthesis; cholecalciferol biosynthesis.
    • Involvement in disease
      Cerebrotendinous xanthomatosis
    • Sequence similarities
      Belongs to the cytochrome P450 family.
    • Cellular localization
      Mitochondrion membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 12-alpha-triol 27-hydroxylase antibody
      • 5-beta-cholestane-3-alpha antibody
      • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
      • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody
      • 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody
      • 7-alpha antibody
      • Cholestanetriol 26 monooxygenase antibody
      • CP27 antibody
      • CP27A_HUMAN antibody
      • CTX antibody
      • CYP antibody
      • CYP27 antibody
      • CYP27A1 antibody
      • Cytochrome P 450C27/25 antibody
      • Cytochrome P-450C27/25 antibody
      • Cytochrome P450 27 antibody
      • Cytochrome P450 27 mitochondrial antibody
      • Cytochrome P450 family 27 subfamily A member 1 antibody
      • Cytochrome P450 family 27 subfamily A polypeptide 1 antibody
      • Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody
      • mitochondrial antibody
      • Sterol 26 hydroxylase antibody
      • Sterol 26 hydroxylase mitochondrial antibody
      • Sterol 26-hydroxylase antibody
      • Sterol 27 hydroxylase antibody
      • Sterol 27-hydroxylase antibody
      • Vitamin D(3) 25 hydroxylase antibody
      • Vitamin D(3) 25-hydroxylase antibody
      see all

    Images

    • All lanes : Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : Caco 2 lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Mouse liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
    • ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.
    • Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    • ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Liu MX  et al. Dicer1/miR-29/HMGCR axis contributes to hepatic free cholesterol accumulation in mouse non-alcoholic steatohepatitis. Acta Pharmacol Sin 38:660-671 (2017). Read more (PubMed: 28112179) »
    • Soffientini U  et al. Intracellular cholesterol transporters and modulation of hepatic lipid metabolism: Implications for diabetic dyslipidaemia and steatosis. Biochim Biophys Acta 1842:1372-82 (2014). Read more (PubMed: 25014273) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 04 2017

    Application
    Western blot
    Loading amount
    100000 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
    Specification
    rat vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 23 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)
    Sample
    Human Tissue sections (aortic atherosclerotic lesion)
    Specification
    aortic atherosclerotic lesion
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 07 2014

    Thank you for contacting Abcam.
    We have now added the blocking peptide for ab126785 to the catalogue. It is under the catalogue number of ab139504:

    http://www.abcam.com/CYP27A1-peptide-ab139504.html



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    I have seen the correspondence you have had with my colleague Claudia over the ab4360 slide, she wrote the following:

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    1. Yes, rabbit polyclonal IgG isotype ab27478 would be an appropriate isotype control for the experiment you are hoping to perform with anti- Cytochro...

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