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Synthetic peptide corresponding to Human CYP27B1 aa 482-508 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: 1594
Our Abpromise guarantee covers the use of ab95047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CYP27B1 with ab95047. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
Flow Cytometry analysis of 293 cells labelling CYP27B1 with ab95047 (green) compared to a negative control (blue). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the primary antibody (1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody, a FITC conjugated goat anti-rabbit IgG, was used at 1/200 dilution for 40 min at room temperature. Acquisition of >10,000 events was performed.
ab95047 has not yet been referenced specifically in any publications.