Overview

  • Product nameAnti-CYP2R1 antibody
    See all CYP2R1 primary antibodies
  • Description
    Rabbit polyclonal to CYP2R1
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C terminal region of human CYP2R1.

  • Positive control
    • NCI-H460 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 57 kDa.
ELISA 1/1000.

Target

  • FunctionHas a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3).
  • Involvement in diseaseDefects in CYP2R1 are the cause of rickets vitamin D-dependent type 1B (VDDR1B) [MIM:600081]; also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CP2R1_HUMAN antibody
    • CYP2R1 antibody
    • Cytochrome P450 2R1 antibody
    • Cytochrome P450 family 2 R1 antibody
    • Cytochrome P450 family 2 subfamily R polypeptide 1 antibody
    • EC 1.14.13.15 antibody
    • MGC4663 antibody
    • Vitamin D 25 hydroxylase antibody
    • Vitamin D 25-hydroxylase antibody
    see all

Anti-CYP2R1 antibody images

  • Anti-CYP2R1 antibody (ab80101) at 1/50 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 57 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
  • ab80101 at 1/50 dilution staining CYP2R1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-CYP2R1 antibody (ab80101)

This product has been referenced in:
  • O'Shaughnessy PJ  et al. Maternal smoking and fetal sex significantly affect metabolic enzyme expression in the human fetal liver. J Clin Endocrinol Metab 96:2851-60 (2011). WB ; Human . Read more (PubMed: 21715529) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"