Overview

  • Product name
    Anti-CYP2U1 antibody
  • Description
    Rabbit polyclonal to CYP2U1
  • Specificity
    Detects endogenous levels of total CYP2U1 protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) from an internal sequence

  • Positive control
    • HeLa and LOVO cell extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab65128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

Images

  • All lanes : Anti-CYP2U1 antibody (ab65128) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : LOVO cell extract
    Lane 3 : LOVO cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 28 kDa,55 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65128 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65128, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab65128 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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