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Rabbit polyclonal to CYP3A43
Predicted to work with:
Recombinant fragment corresponding to a region within amino acids 33-248 of Human CYP3A43 (UniProt ID: Q9HB55).
HCT116, HepG2, HeLa and A549 whole cell lysate. HeLa cells. Human breast carcinoma tissue.
Keep as concentrated solution.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000.
CYP3A43 is a member of the cytochrome P450 superfamily of enzymes which are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP3A43 exhibits low testosterone 6 beta hydroxylase activity.
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Cytochrome P450 family 3 subfamily A polypeptide 43 antibody
Cytochrome P450 polypeptide 43 antibody
Cytochrome P450 subfamily IIIA polypeptide 43 antibody
Western blot - Anti-CYP3A43 antibody (ab155029)
Anti-CYP3A43 antibody (ab155029) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 58 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-CYP3A43 antibody (ab155029)
Immunofluorescence analysis of methanol fixed HeLa cells, labeling CYP3A43 using ab155029 at 1/200 dilution. Lower image costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A43 antibody (ab155029)
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling CYP3A43 using ab155029 at 1/250 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"