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Rabbit polyclonal to CYP3A43 - N-terminal
A synthetic peptide selected from the N terminal region of human CYP3A43 conjugated to KLH (NP_476436)
WB: 293 cell line lysates.
IHC-P: Human prostate carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Ammonium Sulphate Precipitation
ab82849 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
Use at an assay dependent concentration.
CYP3A43 is a member of the cytochrome P450 superfamily of enzymes which are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP3A43 exhibits low testosterone 6 beta hydroxylase activity.
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Cytochrome P450 family 3 subfamily A polypeptide 43 antibody
Cytochrome P450 polypeptide 43 antibody
Cytochrome P450 subfamily IIIA polypeptide 43 antibody
Western blot - CYP3A43 antibody - N-terminal (ab82849)
Anti-CYP3A43 antibody - N-terminal (ab82849) at 1/50 dilution + 293 cell line lysates at 35 µg
Predicted band size: 58 kDa Observed band size: 58 kDa Additional bands at: 47 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CYP3A43 antibody - N-terminal (ab82849)
ab82849 staining CYP3A43 in human prostata carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
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