Overview

  • Product nameAnti-CYP4B1 antibody
    See all CYP4B1 primary antibodies
  • Description
    Rabbit polyclonal to CYP4B1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide (SWAHQFPYAH PLWFGQFIGF LNIYEPDYAK AVYSRGDPKA PDVYDFFLQW) corresponding to a region within N terminal amino acids 72-121 of human CYP4B1 (NP_000770).

  • Positive control
    • Fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Tissue specificityDetected in the liver and lung (at protein level).
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CP4B1_HUMAN antibody
    • CYP4B1 antibody
    • CYPIVB1 antibody
    • Cytochrome P450 4B1 antibody
    • Cytochrome P450 family 4 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily IVB polypeptide 1 antibody
    • Cytochrome P450-HP antibody
    • Microsomal monooxygenase antibody
    • P450HP antibody
    see all

Anti-CYP4B1 antibody images



  • Predicted band size : 59 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling CYP4B1 with ab83224 at 1.0µg/ml.


  • Predicted band size : 59 kDa
    Western blot analysis of Human fetal liver tissue lysate labeling CYP4B1 with ab83224 at 1.0µg/ml.
  • Anti-CYP4B1 antibody (ab83224) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Gel Concentration: 12%

References for Anti-CYP4B1 antibody (ab83224)

ab83224 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"