Overview

  • Product nameAnti-CYP4X1 antibody
    See all CYP4X1 primary antibodies
  • Description
    Rabbit polyclonal to CYP4X1
  • Specificityab74810 detects endogenous levels of total CYP4X1 protein.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from an internal sequence of human CYP4X1.

  • Positive control
    • IHC-P: human liver carcinoma tissue. WB: Extracts from COS7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100.

Target

  • RelevanceCYP4X1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The expression pattern of a similar rat protein suggests that this protein may be involved in neurovascular function in the brain. Catalytic activity: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
  • Cellular localizationEndoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • Database links
  • Alternative names
    • CYPIVX1 antibody
    • Cytochrome P450 4X1 antibody
    • Cytochrome P450, family 4, subfamily X, polypeptide 1 antibody

Anti-CYP4X1 antibody images

  • ab74810, at 1/50 dilution, staining CYP4X1 in human liver carcinoma tissue sections by Immunohistochemistry in the absence (left image) and presence (right image) of the immunising peptide.
  • All lanes : Anti-CYP4X1 antibody (ab74810) at 1/500 dilution

    Lane 1 : extracts from COS78 cells
    Lane 2 : extracts from COS78 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 59 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CYP4X1 antibody (ab74810)

ab74810 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"