Anti-CYP7A1 antibody (ab65596)
Key features and details
- Rabbit polyclonal to CYP7A1
- Suitable for: WB, ELISA
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CYP7A1 antibody
See all CYP7A1 primary antibodies -
Description
Rabbit polyclonal to CYP7A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Dog, Human, Pig, Rhesus monkey -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human CYP7A1.
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Positive control
- WB: High Fat Diet and regular Mouse Liver lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. -
Tissue specificity
Detected in liver. -
Pathway
Lipid metabolism; bile acid biosynthesis. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1581 Human
- Entrez Gene: 13122 Mouse
- Entrez Gene: 25428 Rat
- Omim: 118455 Human
- SwissProt: P22680 Human
- SwissProt: Q64505 Mouse
- SwissProt: P18125 Rat
- Unigene: 1644 Human
see all -
Alternative names
- Cholesterol 7 alpha hydroxylase antibody
- Cholesterol 7 alpha monooxygenase antibody
- Cholesterol 7-alpha-hydroxylase antibody
see all
Images
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All lanes : Anti-CYP7A1 antibody (ab65596) at 1 µg/ml
Lanes 1 & 5 : High Fat Diet Mouse Liver lysate
Lanes 2 & 6 : Mouse Liver lysate
Lanes 3 & 7 : Mouse Thymus lysate
Lanes 4 & 8 : Mouse Colon lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Primary antibody (Green) - ab65596 without (Lanes 1-4) and with (Lanes 5-8) immunising peptide.
Loading Control (Red) - ab8245 at 0.1µg/ml, all lanes.
Secondary antibody - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed at 1/10000 dilution and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed at 1/10000 Dilution.
Blocking Buffer - 3% Milk
Datasheets and documents
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SDS download
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Datasheet download
References (47)
ab65596 has been referenced in 47 publications.
- Hu H et al. Gut microbiota promotes cholesterol gallstone formation by modulating bile acid composition and biliary cholesterol secretion. Nat Commun 13:252 (2022). PubMed: 35017486
- Jeon SH et al. Beneficial Activities of Alisma orientale Extract in a Western Diet-Induced Murine Non-Alcoholic Steatohepatitis and Related Fibrosis Model via Regulation of the Hepatic Adiponectin and Farnesoid X Receptor Pathways. Nutrients 14:N/A (2022). PubMed: 35277054
- Li L et al. Aldehyde dehydrogenase 2 and PARP1 interaction modulates hepatic HDL biogenesis by LXRα-mediated ABCA1 expression. JCI Insight 7:N/A (2022). PubMed: 35393951
- Wang S et al. Hyperoside attenuates non-alcoholic fatty liver disease in rats via cholesterol metabolism and bile acid metabolism. J Adv Res 34:109-122 (2021). PubMed: 35024184
- Li WC et al. Co-administration of obeticholic acid and simvastatin protects against high-fat diet-induced non-alcoholic steatohepatitis in mice. Exp Ther Med 22:830 (2021). PubMed: 34149876