This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).

Target

  • Function
    Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
  • Tissue specificity
    Detected in liver.
  • Pathway
    Lipid metabolism; bile acid biosynthesis.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cholesterol 7 alpha hydroxylase antibody
    • Cholesterol 7 alpha monooxygenase antibody
    • Cholesterol 7-alpha-hydroxylase antibody
    • Cholesterol 7-alpha-monooxygenase antibody
    • CP7A antibody
    • CP7A1_HUMAN antibody
    • CYP 7 antibody
    • CYP7 antibody
    • CYP7A1 antibody
    • CYPVII antibody
    • Cytochrome P450 7A1 antibody
    • Cytochrome P450, family 7, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody
    • MGC126826 antibody
    • MGC138389 antibody
    see all

Images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-CYP7A1 antibody (ab65596) at 1/1000 dilution

    All lanes : Mouse liver lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/5000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 58 kDa
    Additional bands at: 40 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)



    Blocking: 5% Milk

    See Abreview

References

This product has been referenced in:
  • Yetti H  et al. Bile acid detoxifying enzymes limit susceptibility to liver fibrosis in female SHRSP5/Dmcr rats fed with a high-fat-cholesterol diet. PLoS One 13:e0192863 (2018). WB ; Rat . Read more (PubMed: 29438418) »
  • Yuan Y  et al. Combination of Hypertension Along with a High Fat and Cholesterol Diet Induces Severe Hepatic Inflammation in Rats via a Signaling Network Comprising NF-?B, MAPK, and Nrf2 Pathways. Nutrients 9:N/A (2017). WB ; Rat . Read more (PubMed: 28906458) »

See all 11 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jul 05 2017

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Rat Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5%
Username

Abcam user community

Verified customer

Submitted Sep 09 2013



ab65596 es un llamado “Fast Track” antibody. Se trata de anticuerpos que no se han caracterizado completamente, y que se han añadido al catálogo antes de realizar la validación completa por la necesidad urgent...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hearthe customer hashad difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigat...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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