Anti-CYP7A1 antibody - C-terminal (ab79847)

Overview

  • Product name
    Anti-CYP7A1 antibody - C-terminal
    See all CYP7A1 primary antibodies
  • Description
    Rabbit polyclonal to CYP7A1 - C-terminal
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH from the C-terminus

  • Positive control
    • K562 cell lysate

Applications

Our Abpromise guarantee covers the use of ab79847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

  • Function
    Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
  • Tissue specificity
    Detected in liver.
  • Pathway
    Lipid metabolism; bile acid biosynthesis.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cholesterol 7 alpha hydroxylase antibody
    • Cholesterol 7 alpha monooxygenase antibody
    • Cholesterol 7-alpha-hydroxylase antibody
    • Cholesterol 7-alpha-monooxygenase antibody
    • CP7A antibody
    • CP7A1_HUMAN antibody
    • CYP 7 antibody
    • CYP7 antibody
    • CYP7A1 antibody
    • CYPVII antibody
    • Cytochrome P450 7A1 antibody
    • Cytochrome P450, family 7, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody
    • MGC126826 antibody
    • MGC138389 antibody
    see all

Anti-CYP7A1 antibody - C-terminal images

  • Anti-CYP7A1 antibody - C-terminal (ab79847) at 1/50 dilution + K562 cell lysates at 35 µg

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 30 kDa,38 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CYP7A1 antibody - C-terminal (ab79847)

This product has been referenced in:
  • Cai D  et al. Maternal Betaine Supplementation throughout Gestation and Lactation Modifies Hepatic Cholesterol Metabolic Genes in Weaning Piglets via AMPK/LXR-Mediated Pathway and Histone Modification. Nutrients 8: (2016). Read more (PubMed: 27763549) »
  • Hu Y  et al. In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks. PLoS One 10:e0122643 (2015). WB ; Chicken . Read more (PubMed: 25860502) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver - Hepatocellular carcinoma and normal adjace)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid 10mM pH6
Permeabilization
No
Specification
Liver - Hepatocellular carcinoma and normal adjace
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 29 2017

I have asked the lab to arrange for the blocking peptide to be made available. They have informed me that this should be on the catalog tomorrow with the number ab172639 and name Human CYP7A1 peptide.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up